The Department of Biochemistry was set up as an independent and full-fledged department during the year 1925 with Dr V.K. Narayana Menon as the first professor. The Biochemistry department was part of the Physiology department for several years unlike the other colleges where these two departments function individually. In the department of Biochemistry, students have experimented on various topics and performed several projects on hemoglobinopathies, iodine deficiency and pollution, which were carried out successfully at the department. The students also produced several papers. The department has a museum of its own. The department provides Biochemical services to King George Hospital and surrounding Teaching Hospitals.
The 24-hour clinical laboratory is a recent addition to the department with conventional and specialised tests.

PRIZE: Rao Bahadur Dr V.K. Narayana Menon Medal is awarded to meritorious students chosen by way of competitive examination.

Dr Venkateswarulu Prize, Dr M.V.V. Krishna Mohan Memorial Prize and Dr C. Sita Devi University Medal is awarded to meritorious students who have excelled in the subject of Biochemistry.